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TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, <t>CTR-1,</t> FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001
Ctr 1 Abmart, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders"

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

Journal: Molecular Neurobiology

doi: 10.1007/s12035-026-05692-4

TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001
Figure Legend Snippet: TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Techniques Used: Expressing, Western Blot, Comparison, Fluorescence, Immunocytochemistry

Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001
Figure Legend Snippet: Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Techniques Used: Disruption, Functional Assay, Comparison, Expressing, Knockdown, Control, Western Blot, Quantitative RT-PCR, Fluorescence



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Abmart Inc ctr 1 abmart
TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, <t>CTR-1,</t> FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001
Ctr 1 Abmart, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctr 1 abmart/product/Abmart Inc
Average 86 stars, based on 1 article reviews
ctr 1 abmart - by Bioz Stars, 2026-06
86/100 stars
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TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Expressing, Western Blot, Comparison, Fluorescence, Immunocytochemistry

Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Disruption, Functional Assay, Comparison, Expressing, Knockdown, Control, Western Blot, Quantitative RT-PCR, Fluorescence